# README

## Name of Dataset: hemolysis

## Description

This data package contains data and code supporting the work:
Hemolytic toxicity of Prymnesium parvum (B-type) reveals species-specific differences in freshwater fish

The dataset investigates the hemolytic effects of P. parvum following the 2022 Oder River disaster. An experiment was performed to determine differences in hemolytic susceptibility between fish species when exposed to P. parvum.     

## Creators or Authors

Margie Glenna,b*
Sascha Behrensa
Jens Nejstgaarda
Sven Wuertza
Joern Gessnera

aLeibniz Institute of Freshwater Ecology and Inland Fisheries (IGB), Mggelseedamm 310, 12587 Berlin, Germany
bHumboldt-Universitt zu Berlin, Faculty of Life Sciences, Unter den Linden 6, 10099 Berlin, Germany

## Dataset Context 

The dataset was collected for the purpose of investigating the hemolytic effects of P. parvum on fish red blood cells. Red blood cells from five different fish species were exposed at five different concentrations of P. parvum and the remaining blood cells were quantified over four time points. We hypothesized that hemolytic susceptibility to P. parvums toxic effects would vary depending on the species origin of red blood cells. For full methodological details and interpretation of results, please see the publication https://doi.org/10.1016/j.ecoenv.2025.118928.

0147-6513/ 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). 

## File Format and Data Structure

Raw data is stored in one xlsx file named data which includes several variables separated into columns. Variables are described in detail in the accompanying data dictionary. R code for the statistical analysis of the data is stored in one R script named script includes comments to describe each step performed.   

## Contact Information

Margie Glenn
margaret.glenn@igb-berlin.de
margie.r.glenn@gmail.com


